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A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".
Deadline for manuscript submissions: 30 July 2024 | Viewed by 2013
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Special Issue Editor
1. Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China
Interests: crop genetics and biotechnology; molecular interaction between crop and pathogen; gene mining and function identification; molecular breeding
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Biotic and abiotic stresses limit variety improvement and cultivation regulation of crops, including but not limited to rice, corn, sorghum, wheat, cotton, rape, peanut, soybean, sugarcane and beet, and thus seriously restrict the corresponding industrial developments. The response and adaptation mechanisms of crops to these stresses have remained unclear. Focusing our research efforts on crop stress biology and molecular breeding through genetic, genomics, molecular biology and other approaches is therefore vitally important. Current studies in this field seek to reveal the biological basis and processes of important crop traits, including genomics, genes and gene networks; to elucidate the signal transduction pathway of crop stress responses and its interaction mechanism with corresponding stress factors; to elaborate upon the adaptation mechanisms of crops in response to their environment (stress); and to discover and identify key genes and interacting proteins that regulate the formation of traits or adapt to the environment. The exploration of these areas will no doubt reveal gene elements or targets that can be utilized for genetic improvement of important agronomic traits and assist in enhancing crop characteristics or even creating novel germplasms.
This Special Issue, “Crop Stress Biology and Molecular Breeding 4.0”, welcomes original research and review papers considering biological process analysis of important crop traits, crop gene cloning and functional identification, the screening of linkage markers for crop target traits and all other related processes. Papers detailing bioinformatics tools and databases used for crop stress biology and molecular breeding research are also welcomed.
This Special Issue is supervised by Prof. Dr. Youxiong Que and assisted by our Topical Advisory Panel Member Dr. Qibin Wu (National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Sanya/Haikou, 572024/571737, China).
Prof. Dr. Youxiong Que
Guest Editor
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Keywords
- biotic and abiotic stress
- variety improvement
- crop stress biology
- molecular breeding
- biological basis
- signal transduction pathway
- agronomic traits
- excellent germplasm
Supplementary material:
Supplementary File 1 (ZIP, 420 KiB)
19 pages, 21031 KiB
Open AccessArticle
Tobacco Transcription Factor NtWRKY70b Facilitates Leaf Senescence via Inducing ROS Accumulation and Impairing Hydrogen Sulfide Biosynthesis
byXinshuang Zhang, Yan Sun, Hao Wu, Ying Zhu, Xin Liu and Songchong Lu
Int. J. Mol. Sci. 2024, 25(7), 3686; https://doi.org/10.3390/ijms25073686 - 26 Mar 2024
Viewed by 619
Abstract
Leaf senescence is the terminal stage of leaf development, and its initiation and progression are closely controlled by the integration of a myriad of endogenous signals and environmental stimuli. It has been documented that WRKY transcription factors (TFs) play essential roles in regulating [...] Read more.
Leaf senescence is the terminal stage of leaf development, and its initiation and progression are closely controlled by the integration of a myriad of endogenous signals and environmental stimuli. It has been documented that WRKY transcription factors (TFs) play essential roles in regulating leaf senescence, yet the molecular mechanism of WRKY-mediated leaf senescence still lacks detailed elucidation in crop plants. In this study, we cloned and identified a tobacco WRKY TF gene, designated NtWRKY70b, acting as a positive regulator of natural leaf senescence. The expression profile analysis showed that NtWRKY70b transcript levels were induced by aging and hydrogen peroxide (H2O2) and downregulated upon hydrogen sulfide (H2S) treatment. The physiological and biochemical assays revealed that overexpression of NtWRKY70b (OE) clearly promoted leaf senescence, triggering increased levels of reactive oxygen species (ROS) and decreased H2S content, while disruption of NtWRKY70b by chimeric repressor silencing technology (SRDX) significantly delayed the onset of leaf senescence, leading to a decreased accumulation of ROS and elevated concentration of H2S. The quantitative real-time PCR analysis showed that the expression levels of various senescence-associated genes and ROS biosynthesis-related genes (NtRbohD and NtRbohE) were upregulated in OE lines, while the expression of H2S biosynthesis-related genes (NtDCD and NtCYSC1) were inhibited in OE lines. Furthermore, the Yeast one-hybrid analysis (Y1H) and dual luciferase assays showed that NtWRKY70b could directly upregulate the expression of an ROS biosynthesis-related gene (NtRbohD) and a chlorophyll degradation-related gene (NtPPH) by binding to their promoter sequences. Accordingly, these results indicated that NtWYKY70b directly activated the transcript levels of NtRbohD and NtPPH and repressed the expression of NtDCD and NtCYCS1, thereby promoting ROS accumulation and impairing the endogenous H2S production, and subsequently accelerating leaf aging. These observations improve our knowledge of the regulatory mechanisms of WRKY TFs controlling leaf senescence and provide a novel method for ensuring high agricultural crop productivity via genetic manipulation of leaf senescence in crops.Full article
(This article belongs to the Special Issue Crop Stress Biology and Molecular Breeding 4.0)
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